Order SeqName SeqType IsAE NBL01_MYCN NBL05_MYCN NBL09_MYCN NBL10_MYCN NBL02 NBL03 NBL04 NBL06 NBL07 NBL08 NBL11_Rel2 NBL11_Rem1 NBL11_Rel1 NBL12_Rel_Left NBL12_Rel_Right NBL13_Rel_Left NBL13_Rel_Right NBL14_MYCN_Met NBL14_MYCN_Pri NBL15_MYCN_Rel1 SKP01 SKP02 SKP03 1 MYO5B Gene 0 2.40 23.40 3.05 4.10 3.49 2.80 3.04 2.52 3.61 3.05 2.30 4.02 2.66 5.07 0.84 0.68 1.31 20.80 1.99 3.19 10.62 24.11 19.08 2 324581 Transcript 0 0.00 19.01 0.00 0.00 3.74 0.00 0.00 0.00 4.20 0.00 0.00 0.00 0.00 2.24 0.00 0.00 0.00 18.65 0.00 0.00 9.82 22.60 11.01 3 285039 Transcript 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 356732 Transcript 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5 ER1a ExonRegion 0 0.00 1.63 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.00 0.13 0.00 0.00 1.28 0.00 0.00 0.00 2.21 0.00 0.00 2.22 7.11 2.97 6 E1a_E3a KnownJunction 0 0.00 6.45 0.00 0.00 0.00 0.00 1.68 0.00 1.16 0.00 2.53 0.00 0.00 0.00 0.00 0.00 0.00 8.46 0.00 0.00 2.41 19.96 3.76 7 ER2a ExonRegion 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 8 ER3a ExonRegion 0 0.00 9.97 0.00 0.00 0.00 0.00 0.00 0.00 1.25 0.00 1.69 0.00 0.00 2.11 0.00 0.00 0.00 7.09 0.00 0.00 17.53 21.42 14.00 9 E3a_E4a KnownJunction 0 0.00 20.06 0.00 0.00 0.00 0.00 0.00 0.00 3.10 0.00 2.42 0.00 0.00 4.65 0.00 0.00 0.00 24.25 0.00 0.00 24.59 29.70 22.96 10 ER4a ExonRegion 0 0.00 12.52 0.00 0.00 0.00 0.00 0.00 0.77 2.36 0.00 2.48 1.28 1.15 0.99 0.00 0.00 1.96 26.62 0.00 0.63 24.86 33.81 30.00 11 E4a_E5a KnownJunction 0 0.00 18.29 0.00 0.00 0.00 0.00 0.00 4.15 3.59 0.00 2.68 0.00 0.00 0.00 0.00 0.00 0.00 27.32 0.00 0.00 15.01 30.92 11.95 12 ER5a ExonRegion 0 0.74 14.38 0.00 0.00 0.44 0.00 0.00 1.54 1.62 0.32 3.27 1.52 0.36 0.00 0.00 0.00 2.69 31.82 0.00 0.75 17.25 25.59 25.60 13 E5a_E6a KnownJunction 0 0.00 29.53 0.00 0.00 4.33 0.00 0.00 4.03 3.07 1.67 4.79 0.00 6.82 0.00 0.00 0.00 4.21 27.57 0.00 0.00 13.78 28.90 32.69 14 ER6a ExonRegion 0 0.00 13.78 0.00 0.00 0.46 0.00 0.86 0.24 1.09 0.64 2.40 0.00 0.93 0.43 0.00 0.00 0.74 22.14 0.00 0.69 11.96 21.54 25.61 15 E6a_E7a KnownJunction 0 0.00 24.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.50 2.37 0.00 0.00 0.00 0.00 0.00 4.21 41.04 0.00 0.00 20.76 36.07 27.23 16 ER7a ExonRegion 0 0.00 11.65 0.00 0.00 0.00 0.00 0.00 0.91 1.15 0.45 4.26 0.00 0.00 1.23 0.00 0.00 0.00 23.17 0.00 0.75 15.47 27.52 22.70 17 E7a_E8a KnownJunction 0 0.00 25.39 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.67 7.05 0.00 0.00 2.47 0.00 0.00 0.00 33.83 0.00 0.00 20.94 27.56 26.38 18 ER8a ExonRegion 0 1.32 12.70 0.00 0.00 0.00 0.00 0.00 0.86 0.00 0.63 2.73 0.00 0.00 0.34 0.00 0.00 1.49 38.15 0.00 0.00 13.89 29.14 24.64 19 E8a_E9a KnownJunction 0 0.00 11.45 0.00 0.00 0.00 0.00 0.00 2.55 0.00 0.00 4.90 0.00 0.00 0.00 0.00 0.00 4.21 26.41 0.00 0.00 11.31 15.37 19.29 20 ER9a ExonRegion 0 0.63 22.41 0.00 0.00 0.00 0.00 0.00 1.78 0.00 0.81 4.85 0.00 0.00 0.98 0.00 0.00 1.59 24.89 0.00 0.00 13.39 31.04 27.00 21 E9a_E10a KnownJunction 0 3.70 23.88 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.47 0.00 0.00 4.11 38.34 0.00 0.00 10.50 28.53 44.13 22 ER10a ExonRegion 0 0.37 12.90 0.00 0.00 1.24 0.00 0.23 1.19 0.00 0.00 3.08 0.00 0.00 0.28 0.00 0.00 0.39 28.09 0.00 0.00 12.40 48.64 32.54 23 E10a_E11a KnownJunction 0 0.00 26.25 0.00 0.00 0.00 0.00 2.21 1.56 0.00 0.00 2.53 0.00 0.00 0.00 0.00 0.00 0.00 20.35 0.00 0.00 9.45 35.45 67.44 24 ER11a ExonRegion 0 0.21 16.82 0.00 0.00 0.00 0.00 0.41 1.48 0.22 0.26 3.47 0.00 0.00 0.00 0.00 0.00 0.22 21.58 0.00 0.00 14.70 32.26 38.75 25 E11a_E12a KnownJunction 0 3.78 18.22 0.00 0.00 0.00 0.00 0.00 0.00 1.74 1.24 4.37 0.00 0.00 0.00 0.00 0.00 4.58 23.88 0.00 0.00 32.37 53.27 47.97 26 ER12a ExonRegion 0 1.94 12.80 0.00 0.00 0.00 0.00 0.82 0.00 1.79 1.96 3.25 0.00 0.00 0.38 1.03 0.00 1.33 36.73 0.00 0.00 17.52 34.35 43.37 27 E12a_E13a KnownJunction 0 3.48 13.94 0.00 0.00 0.00 0.00 0.00 0.00 2.95 3.06 7.26 0.00 0.00 2.88 0.00 0.00 0.00 49.83 0.00 0.00 13.41 16.41 54.72 28 ER13a ExonRegion 0 0.00 23.00 0.00 0.00 0.00 0.00 0.00 0.88 1.21 1.96 7.03 0.00 0.00 1.29 0.56 0.00 1.19 36.56 0.00 0.00 20.35 45.17 42.05 29 E13a_E14a KnownJunction 0 0.00 27.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.21 4.42 0.00 0.00 0.00 4.45 0.00 0.00 44.23 0.00 0.00 22.49 42.62 40.46 30 ER14a ExonRegion 0 0.00 16.67 0.00 0.00 0.00 0.00 0.55 0.51 0.43 0.54 4.21 0.00 0.00 10.71 1.23 0.00 0.00 51.86 0.00 0.00 25.46 39.69 50.78 31 E14a_E15a KnownJunction 0 0.00 11.91 0.00 0.00 0.00 0.00 0.00 2.55 3.04 1.67 9.11 0.00 0.00 20.37 9.77 0.00 0.00 77.02 0.00 0.00 23.04 59.95 47.29 32 ER15a ExonRegion 0 0.00 16.90 0.00 0.00 0.00 0.00 0.00 0.91 2.04 0.44 5.71 0.00 0.00 7.79 4.25 0.00 2.00 55.43 0.00 0.00 27.94 38.12 49.33 33 E15a_E16a KnownJunction 0 0.00 13.88 0.00 0.00 0.00 0.00 0.00 0.00 1.77 0.00 4.53 0.00 0.00 14.83 0.00 0.00 0.00 48.67 0.00 0.00 22.18 21.86 60.52 34 ER16a ExonRegion 0 1.41 20.02 0.00 0.00 0.00 0.61 0.00 0.00 2.19 0.66 4.54 0.00 0.00 9.68 1.65 0.00 1.10 49.71 0.00 0.00 24.93 40.59 39.06 35 E16a_E17a KnownJunction 0 0.00 23.75 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.16 6.37 0.00 0.00 5.30 0.00 0.00 0.00 62.10 0.00 0.00 19.95 22.72 23.47 36 ER17a ExonRegion 0 0.35 19.51 0.00 0.00 1.39 2.51 0.00 0.00 2.42 0.75 2.29 0.00 0.00 9.28 1.07 0.00 2.73 39.05 0.00 0.00 19.36 29.47 46.57 37 E17a_E18a KnownJunction 0 3.48 17.37 0.00 0.00 0.00 5.68 0.00 0.00 3.04 0.00 0.00 0.00 0.00 7.42 0.00 0.00 4.77 26.78 0.00 0.00 8.34 17.82 16.82 38 ER18a ExonRegion 0 0.85 10.92 0.00 0.00 0.00 0.40 1.55 0.00 2.31 0.00 1.13 0.00 0.00 7.50 0.00 0.00 0.80 24.47 0.00 0.00 15.05 18.44 15.36 39 E18a_E19a KnownJunction 0 0.00 15.39 0.00 0.00 0.00 0.00 0.00 0.00 1.71 0.00 0.00 0.00 0.00 2.41 0.00 0.00 0.00 20.10 0.00 0.00 5.25 29.51 12.38 40 ER19a ExonRegion 0 0.00 14.05 0.00 0.92 1.22 0.69 0.00 0.00 1.99 0.00 3.37 0.00 0.00 6.82 0.54 0.00 3.01 26.54 1.45 0.00 13.50 19.96 18.64 41 E19a_E20a KnownJunction 0 0.00 19.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.11 0.00 0.00 11.60 4.45 0.00 0.00 20.10 0.00 0.00 12.48 19.29 28.43 42 ER20a ExonRegion 0 0.00 11.10 0.00 0.00 0.00 0.08 0.00 0.62 1.72 0.96 2.28 0.00 0.00 6.34 1.68 0.00 0.79 22.53 0.77 0.00 8.62 18.94 23.44 43 E20a_E21a KnownJunction 0 0.00 9.14 0.00 0.00 0.00 0.00 0.00 0.00 3.12 1.50 2.37 0.00 0.00 7.12 0.00 0.00 0.00 23.55 0.00 0.00 10.56 19.53 20.49 44 ER21a ExonRegion 0 1.11 7.25 0.00 0.00 0.87 0.00 0.00 0.00 2.75 0.93 1.43 0.00 0.00 4.94 1.33 0.00 2.14 13.73 0.00 0.00 10.36 13.24 15.77 45 E21a_E22a KnownJunction 0 3.78 6.25 0.00 0.00 0.00 0.00 0.00 0.00 2.89 0.00 0.00 0.00 0.00 4.71 0.00 0.00 0.00 15.55 0.00 0.00 4.76 19.59 15.02 46 ER22a ExonRegion 0 0.18 6.91 0.00 0.00 0.00 1.56 0.00 2.33 1.10 1.75 2.80 0.00 0.00 6.03 0.68 0.00 1.68 15.83 0.00 0.00 13.98 17.29 15.33 47 E22a_E23a KnownJunction 0 0.00 15.33 0.00 0.00 3.48 0.00 0.00 2.09 1.56 5.75 5.32 0.00 0.00 2.47 4.45 0.00 4.77 30.63 0.00 0.00 3.15 21.80 22.88 48 ER23a ExonRegion 0 0.00 18.02 0.00 0.00 0.00 0.00 0.29 1.61 2.83 2.68 3.02 0.00 0.00 5.49 0.20 0.00 1.16 20.81 0.00 0.00 11.65 25.11 14.93 49 E23a_E24a KnownJunction 0 0.00 5.66 0.00 0.00 0.00 0.00 0.00 1.68 4.75 0.00 0.00 0.00 0.00 9.77 0.00 0.00 0.00 25.41 0.00 0.00 28.36 58.78 29.45 50 ER24a ExonRegion 0 1.69 17.18 0.00 0.00 1.61 0.00 0.00 2.89 3.11 0.23 3.96 0.00 0.00 7.59 0.00 0.00 3.34 27.67 0.00 0.00 21.16 30.66 38.42 51 E24a_E25a KnownJunction 0 7.04 45.52 0.00 0.00 0.00 0.00 0.00 0.00 3.56 1.29 4.69 0.00 0.00 7.06 0.00 0.00 4.39 25.66 0.00 0.00 17.24 36.19 18.95 52 ER25a ExonRegion 0 1.97 28.94 0.00 0.71 0.00 0.58 0.24 0.54 1.81 0.60 3.14 0.00 0.00 6.25 0.00 0.00 0.36 22.93 0.00 0.00 14.67 40.13 28.80 53 E25a_E26a KnownJunction 0 0.00 46.24 0.00 0.00 0.00 5.74 2.25 2.46 0.00 1.32 2.21 0.00 0.00 15.66 0.00 0.00 0.00 39.51 0.00 0.00 19.34 20.39 49.51 54 ER26a ExonRegion 0 0.00 35.21 0.00 0.39 0.00 1.64 0.26 0.43 0.04 0.55 4.27 0.00 0.00 6.42 0.00 0.00 1.73 39.71 0.00 0.00 19.65 38.44 28.85 55 E26a_E27a KnownJunction 0 0.00 46.64 0.00 2.06 0.00 0.00 0.00 0.00 0.00 0.00 2.32 0.00 0.00 2.47 0.00 0.00 4.02 37.23 0.00 0.00 19.46 44.21 19.89 56 ER27a ExonRegion 0 0.96 27.26 0.00 0.41 0.97 0.00 0.48 0.74 1.58 0.81 3.17 0.00 0.00 8.02 0.00 0.00 2.11 34.41 2.30 0.75 17.50 35.74 24.18 57 E27a_E28a KnownJunction 0 3.26 14.21 0.00 0.00 0.00 0.00 0.00 4.60 2.92 1.48 1.84 0.00 0.00 4.71 0.00 0.00 0.00 35.19 0.00 0.00 7.97 38.82 32.35 58 ER28a ExonRegion 0 2.18 16.05 0.00 0.47 2.07 2.95 0.00 2.41 1.40 1.49 1.55 0.00 0.00 5.56 0.00 0.00 2.55 26.18 3.57 0.03 11.55 36.21 19.18 59 E28a_E29a KnownJunction 0 3.78 20.98 0.00 1.80 0.00 0.00 0.00 0.00 1.79 1.67 2.11 0.00 0.00 4.89 0.00 0.00 0.00 25.66 12.71 0.00 15.20 35.03 11.61 60 ER29a ExonRegion 0 1.53 19.79 0.78 0.73 0.00 1.45 0.00 1.09 1.21 1.06 1.53 0.00 0.00 5.04 0.44 0.00 1.40 30.82 0.37 0.00 12.38 26.03 16.19 61 E29a_E30a KnownJunction 0 0.00 13.09 0.00 0.00 0.00 11.58 0.00 0.00 1.79 1.56 4.47 0.00 0.00 2.47 0.00 0.00 0.00 55.30 0.00 0.00 16.87 14.63 22.62 62 ER30a ExonRegion 0 1.07 26.92 0.00 0.00 1.19 5.16 0.00 1.30 1.85 0.56 2.39 0.00 0.00 7.62 2.24 0.00 1.67 41.55 0.00 0.00 22.93 28.90 17.65 63 E30a_E31a KnownJunction 0 0.00 0.00 0.00 0.00 0.00 2.84 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.57 0.00 0.00 0.00 0.00 0.00 64 E30a_E32a KnownJunction 0 0.00 19.01 0.00 0.00 3.74 0.00 0.00 0.00 4.20 0.00 0.00 0.00 0.00 2.24 0.00 0.00 0.00 18.65 0.00 0.00 9.82 22.60 11.01 65 ER31a ExonRegion 0 0.00 0.08 0.00 0.00 0.00 0.75 0.00 1.15 0.00 0.00 0.00 0.00 0.00 0.06 0.00 0.00 0.00 0.97 0.00 0.00 0.00 1.34 1.81 66 E31a_E32a KnownJunction 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.26 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.53 0.00 0.00 0.00 0.00 0.00 67 ER32a ExonRegion 0 2.28 21.90 0.00 0.00 2.35 4.26 0.00 0.87 2.98 0.95 2.29 0.15 0.00 5.22 0.61 0.00 0.27 49.04 0.82 0.00 15.65 28.06 22.06 68 E32a_E33a KnownJunction 0 3.04 31.70 0.00 0.00 3.99 5.79 0.00 0.00 1.30 1.16 4.90 0.00 0.00 4.94 0.00 0.00 4.39 28.02 0.00 0.00 14.89 22.72 38.41 69 ER33a ExonRegion 0 4.30 27.30 0.00 0.00 4.50 0.94 0.00 1.71 2.12 1.71 3.27 2.03 0.00 7.46 0.00 0.00 6.60 40.65 0.00 0.00 17.91 41.47 32.90 70 E33a_E34a KnownJunction 0 13.11 25.92 0.00 0.00 4.33 0.00 0.00 4.97 4.95 0.00 2.32 0.00 0.00 16.72 0.00 0.00 0.00 53.23 0.00 0.00 13.96 38.64 27.91 71 ER34a ExonRegion 0 1.54 28.46 0.00 0.00 0.56 0.00 0.40 3.24 2.83 1.52 4.32 0.00 0.00 7.58 2.18 0.00 4.68 38.64 1.13 0.00 23.61 34.16 25.14 72 E34a_E35a KnownJunction 0 0.00 46.44 3.64 0.00 0.00 0.00 1.72 1.93 5.24 4.92 0.00 0.00 0.00 9.83 0.00 0.00 0.00 23.30 0.00 0.00 16.93 42.07 19.63 73 ER35a ExonRegion 0 1.62 23.67 0.00 0.00 0.00 1.68 1.24 1.08 4.24 3.39 3.54 0.00 0.00 7.56 3.66 0.00 1.11 47.48 1.34 1.43 18.95 30.01 33.23 74 E35a_E36a KnownJunction 0 3.33 8.75 0.00 0.00 0.00 2.56 2.21 0.00 1.39 3.84 5.32 0.00 0.00 11.48 0.00 0.00 0.00 42.37 4.14 0.00 17.98 50.15 39.86 75 ER36a ExonRegion 0 6.75 19.09 1.01 0.05 1.61 0.29 1.09 1.69 1.66 3.91 4.42 0.00 0.00 6.35 3.67 0.00 1.87 40.59 1.34 0.00 17.04 34.05 26.17 76 E36a_E37a KnownJunction 0 0.00 8.81 6.83 0.00 0.00 0.00 0.00 1.73 1.50 13.67 7.11 0.00 0.00 2.47 10.38 0.00 0.00 52.19 0.00 0.00 6.12 14.57 22.71 77 I36_AR1 ActiveIntronRegion 0 11.88 4.44 6.67 14.26 5.32 4.52 3.14 2.90 4.34 6.09 0.30 4.97 1.27 2.82 2.97 0.68 0.51 2.18 4.63 6.18 3.71 1.44 2.25 78 E37_Aa NovelBoundary 0 10.67 0.00 10.76 2.03 11.21 2.84 2.21 0.00 2.49 8.00 0.00 0.00 0.00 2.47 0.00 0.00 0.00 6.80 0.00 2.88 0.00 0.00 3.59 79 ER37a ExonRegion 0 5.07 24.19 4.90 4.90 2.36 3.66 3.99 2.90 4.36 4.20 3.08 0.00 1.30 11.16 2.52 3.01 1.11 36.10 3.23 2.16 14.99 37.13 35.45 80 E37_Da NovelBoundary 0 10.45 3.35 11.44 7.76 0.00 5.28 4.42 2.38 6.34 7.09 2.63 0.00 0.00 0.00 0.00 0.00 0.00 7.63 4.80 8.82 0.00 3.12 0.00 81 E37a_E38a KnownJunction 0 0.00 26.97 1.48 0.00 4.07 0.00 0.00 2.55 1.39 1.37 2.37 0.00 0.00 9.12 5.19 0.00 0.00 51.24 0.00 0.00 0.00 29.27 23.30 82 ER38a ExonRegion 0 5.29 33.78 9.19 6.47 4.93 2.32 2.12 7.90 5.60 5.95 1.63 1.47 1.38 11.91 3.11 0.55 2.35 43.76 5.71 3.41 17.29 34.66 28.71 83 E38_Da NovelBoundary 0 10.59 3.03 8.31 10.45 0.00 0.00 0.00 0.00 4.92 6.66 0.00 0.00 0.00 3.00 0.00 4.10 0.00 13.43 12.80 5.88 5.93 2.51 4.35 84 E38a_E39a KnownJunction 0 7.48 23.42 3.99 0.00 3.65 0.00 0.00 7.35 1.27 6.93 0.00 0.00 0.00 9.77 0.00 0.00 0.00 51.11 0.00 0.00 16.74 52.35 49.77 85 E39_Aa NovelBoundary 0 9.48 6.38 15.67 8.76 7.72 2.21 6.98 2.14 6.63 3.98 2.16 0.00 0.00 4.94 6.30 0.00 0.00 2.57 8.09 12.08 5.75 12.12 10.76 86 ER39a ExonRegion 0 5.90 33.42 4.72 2.94 8.13 6.95 1.36 7.18 6.49 3.01 4.06 1.16 0.00 10.14 0.31 1.70 0.00 41.96 6.48 2.03 28.89 45.28 38.75 87 E39a_E40a KnownJunction 0 3.78 37.56 9.40 0.00 33.96 0.00 8.48 5.91 3.01 11.23 7.69 0.00 0.00 7.30 4.45 0.00 0.00 55.34 3.58 2.49 47.14 85.60 67.01 88 E40_Aa NovelBoundary 0 2.89 12.83 2.99 15.75 11.88 2.90 7.11 6.08 12.62 10.83 7.37 12.60 8.28 4.94 0.00 0.00 4.58 6.26 8.09 11.57 12.05 19.10 17.50 89 ER40a ExonRegion 0 6.17 46.40 2.24 1.18 5.98 4.98 2.06 3.84 7.00 0.81 5.82 0.00 9.73 10.39 0.61 3.74 2.08 37.00 6.92 6.33 25.03 56.56 32.27 90 E40_Da NovelBoundary 0 41.12 9.60 3.40 15.18 22.33 12.15 6.32 9.12 7.81 7.44 0.00 18.91 19.97 11.65 0.00 0.00 0.00 2.11 4.80 3.26 0.00 0.00 4.35 91 E40a_E41a KnownJunction 0 3.33 71.76 3.40 1.33 8.66 2.67 0.00 2.34 0.00 6.18 2.58 0.00 0.00 4.94 0.00 0.00 0.00 35.40 9.22 0.00 21.99 34.90 26.21 92 E41_Aa NovelBoundary 0 3.11 18.35 7.36 11.62 0.00 2.67 5.92 7.31 9.84 8.60 2.63 12.60 0.00 10.36 0.00 0.00 4.02 1.74 0.00 10.93 3.15 2.57 11.61 93 ER41a ExonRegion 0 3.82 30.30 6.27 9.08 7.01 5.23 6.75 3.82 5.84 5.51 1.32 9.52 6.06 4.70 0.80 1.36 1.17 7.16 3.48 7.05 2.68 16.18 8.53