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I~ | /usr/include/usr/include/bits/usr/lib/gcc/x86_64-redhat-linux/4.1.2/includeRNAaliduplex_cmdl.cstdlib.hRNAaliduplex_cmdl.hstdio.hlibio.htypes.hstddef.h J%XuuwuztwyuVLWK:Lf Js=:L>8}Y"]E3yJrrVVVVV)D-"Ʉ =YvLHZY=KI=-:>5/oW? (vt <v< +LY=c<fkK^w<4&:zI' Yvzz&Y z9X@:>4;/[xJ~+KZuIS"YWrf"YWf<p<Xk<vn.Xqy~\~t~tXsh~t_fJ~f^f"z.]~/?{J}}th}<}feglQsue`ztltYs>tgt~Jt~fgutynY{te f!.%|D&|J-~X%fv1"{ ^$<tؑ^)=;u zfz.8{N{3~GHY,t?{U|Dss#!/u~>.#sgudhftte/-$=; .{t>:Ivegf|GX5#J& C{U.)RGYx=]bYZ_a\":>Z_) %s="%s" %s RNAaliduplexhelpdetailed-helpversiondeltaEnergysortedtempnoTetradanglesnoLPnoGUnoClosingGUnoconvnspparamFilew2.0.0%s %s %s %s --%s: option `%s' is ambiguous hVe:sT:4d:P:%s: invalid option -- %c 2%s: option unknown: %c%s General Options: Model Details:%s: cannot dump options to stream %s: `--%s' (`-%c') option given more than once%s %s: `--%s' option given more than once%s %s: invalid numeric value: %s %s: cannot open file for writing: %s %s: option `--%s' doesn't allow an argument %s: option `%c%s' doesn't allow an argument %s: option `%s' requires an argument %s: unrecognized option `--%s' %s: option requires an argument -- %c Predict conserved RNA-RNA interactions between two alignmentsUsage: RNAaliduplex [options] The program reads two alignments of RNA sequences in CLUSTAL format and predicts optimal and suboptimal binding sites, hybridization energies and the corresponding structures. The calculation takes only inter-molecular base pairs into account, for the general case use RNAcofold. The use of alignments allows to focus on binding sites that are evolutionary conserved. Note, that the two input alignments need to have equal number of sequences and the same order, i.e. the 1st sequence in file1 will be hybridized to the 1st in file2 etc. The computed binding sites, energies, and structures are written to stdout, one structure per line. Each line consist of: The structure in dot bracket format with a "&" separating the two strands. The range of the structure in the two sequences in the format "from,to : from,to"; the energy of duplex structure in kcal/mol. The format is especially useful for computing the hybrid structure between a small probe sequence and a long target sequence. -h, --help Print help and exit --detailed-help Print help, including all details and hidden options, and exit -V, --version Print version and exit Below are command line options which alter the general behavior of this program -e, --deltaEnergy=range Compute suboptimal structures with energy in a certain range of the optimum (kcal/mol). Default is calculation of mfe structure only. -s, --sorted print output sorted by free energy (default=off) -T, --temp=DOUBLE Rescale energy parameters to a temperature of temp C. Default is 37C. -4, --noTetra Do not include special tabulated stabilizing energies for tri-, tetra- and hexaloop hairpins. Mostly for testing. (default=off) -d, --dangles=INT How to treat "dangling end" energies for bases adjacent to helices in free ends and multi-loops (default=`2') With -d1 only unpaired bases can participate in at most one dangling end, this is the default for mfe folding but unsupported for the partition function folding. With -d2 this check is ignored, dangling energies will be added for the bases adjacent to a helix on both sides in any case; this is the default for partition function folding (-p). -d0 ignores dangling ends altogether (mostly for debugging). With -d3 mfe folding will allow coaxial stacking of adjacent helices in multi-loops. At the moment the implementation will not allow coaxial stacking of the two interior pairs in a loop of degree 3 and works only for mfe folding. Note that by default (as well as with -d1 and -d3) pf and mfe folding treat dangling ends differently. Use -d2 in addition to -p to ensure that both algorithms use the same energy model. --noLP Produce structures without lonely pairs (helices of length 1). (default=off) For partition function folding this only disallows pairs that can only occur isolated. Other pairs may still occasionally occur as helices of length 1. --noGU Do not allow GU pairs (default=off) --noClosingGU Do not allow GU pairs at the end of helices (default=off) --noconv Do not automatically substitude nucleotide "T" with "U" (default=off) --nsp=STRING Allow other pairs in addition to the usual AU,GC,and GU pairs. Its argument is a comma separated list of additionally allowed pairs. If the first character is a "-" then AB will imply that AB and BA are allowed pairs. e.g. RNAfold -nsp -GA will allow GA and AG pairs. Nonstandard pairs are given 0 stacking energy. -P, --paramFile=paramfile Read energy parameters from paramfile, instead of using the default parameter set. A sample parameter file should accompany your distribution. See the RNAlib documentation for details on the file format. See also: RNAduplex(l) RNAcofold(l) RNAfold(l) Written by: Ivo L Hofacker Report bugs: If in doubt our program is right, nature is at fault. Comments should be sent to rna@tbi.univie.ac.at x $9J[8)$ EHYM&$UADG AA$AHD SN $J^P$wJL nD,A,A<wBBB B(A0A8JOAG0%AzRx $49J[8\)t$ EHYM&UADG AAAHD <TSN $lJ^PwJL nD,A,A4wBBB B(A0A8JLOAG0l%Aww8USLUL]U]F\\VPVLSSLQQQLYYLtYY 7U7FU 2T27Q7ATAFQ -Q-7R7:Q (R7FRPQwQUwU\w\w PsUs)V*NVOlVl~U~VUVPeTe(S*MSOlSlyTyS'P*FPLLPPwwUwwUSwwww UVUPSUww UVUVSS(w( w,U,V VGTG^ ^# QN ` Qk Q Q  Q Q6 F QX e Qe m Qw Q RN ^ Rk R R  R ( R6 N RX e Re m Rw  RPXP\ \3 YN h Yk Y Y  Y ( Y6 N YX e Ye m Yw Y]]]N ]N h Ph ] P ] P  ] ( P( 6 ]6 N PN ]] ]_  _]8]X8X8G XG N 8N h Xh 8 X  8 ( X( 6 86 N XN w 8w X 8]]rTrT@ T@ G YG  T w w T X SSSN S w S w ' w U V V % R T \ ' \ P S ' S V ' VP T wT w w w w  w " w" $ w$ & w& ( w ( ) w() * w0* 4 w84 w r Ur | r Tr | ? 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