/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/tophat -r 336 -o /projects/NBL/HS1195/30TTYAAXX_2/tophat /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat/30TTYAAXX_2_1_export.txt.fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat/30TTYAAXX_2_2_export.txt.fastq
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-microexon-search --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat/30TTYAAXX_2_1_export.txt.fastq
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-microexon-search --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat/30TTYAAXX_2_2_export.txt.fastq
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileiiJKD7
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-microexon-search /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq /dev/null /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads.bwtout  > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filesvf2JT
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg1.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg1.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filev2h1Aw
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg2.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg2.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/file4sVCOg
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filetOORbs
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-closure-search --no-microexon-search /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq /dev/null /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads.bwtout  > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filehxfcj9
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg1.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg1.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filey2Tf9r
bowtie -q --un /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 1 -k 40 -m 40 /projects/rcorbettprj/trans_abyss/bowtie_index/hg18 /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg2.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg2.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filej591zG
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/segment_juncs --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-closure-search --no-microexon-search --ium-reads /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg1_missing.fq,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg2_missing.fq,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg1_missing.fq,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg2_missing.fq /projects/rcorbettprj/trans_abyss/bowtie_index/hg18.fa /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//segment.juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg1.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg2.bwtout /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg1.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg2.bwtout
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/juncs_db 3 25 /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//segment.juncs /projects/rcorbettprj/trans_abyss/bowtie_index/hg18.fa
bowtie-build /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs.fa /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs
bowtie -q -v 2 -p 1 -k 40 -m 40 /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg1.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg1.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filebe7tcU
bowtie -q -v 2 -p 1 -k 40 -m 40 /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg2.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/left_kept_reads_seg2.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filep5HXVF
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-closure-search --no-microexon-search /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//segment.juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg1.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg2.bwtout /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg1_to_spliced.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//left_kept_reads_seg2_to_spliced.bwtout > /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/ /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filesvf2JT /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq.candidate_hits.sam > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filefLLwEt
mv /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filefLLwEt /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq.candidate_hits.sam
bowtie -q -v 2 -p 1 -k 40 -m 40 /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg1.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg1.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/file6idWpW
bowtie -q -v 2 -p 1 -k 40 -m 40 /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/segment_juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg2.fq | /home/rcorbett/aligners/tophat/tophat-1.0.13/bin/fix_map_ordering --fastq /projects/NBL/HS1195/30TTYAAXX_2/tophat//tmp/right_kept_reads_seg2.fq - > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileQSTErj
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-closure-search --no-microexon-search /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//segment.juncs /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg1.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg2.bwtout /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg1_to_spliced.bwtout,/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp//right_kept_reads_seg2_to_spliced.bwtout > /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/ /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/filehxfcj9 /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq.candidate_hits.sam > /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileQjeNwE
mv /projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileQjeNwE /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq.candidate_hits.sam
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir /projects/NBL/HS1195/30TTYAAXX_2/tophat/ --max-multihits 40 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --inner-dist-mean 336 --inner-dist-std-dev 20 --no-closure-search --no-microexon-search junctions.bed accepted_hits.sam /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq.candidate_hits.sam /projects/NBL/HS1195/30TTYAAXX_2/tophat/left_kept_reads.fq /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq.candidate_hits.sam /projects/NBL/HS1195/30TTYAAXX_2/tophat/right_kept_reads.fq
sort -k 3,3 -k 4,4n --temporary-directory=/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/ /projects/NBL/HS1195/30TTYAAXX_2/tophat/accepted_hits.sam > <open file '/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileeL9IdV', mode 'a' at 0x558dab18>
mv <open file '/projects/NBL/HS1195/30TTYAAXX_2/tophat/tmp/fileeL9IdV', mode 'a' at 0x558dab18> /projects/NBL/HS1195/30TTYAAXX_2/tophat/accepted_hits.sam
/home/rcorbett/aligners/tophat/tophat-1.0.13/bin/wiggles /projects/NBL/HS1195/30TTYAAXX_2/tophat/accepted_hits.sam /projects/NBL/HS1195/30TTYAAXX_2/tophat/coverage.wig