FastQCFastQC Report
Thu 14 Mar 2019
ENCFF001HPI_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameENCFF001HPI_trimmed.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16988516
Sequences flagged as poor quality0
Sequence length20-36
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495330.2915675506913023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA8950.012.827064514
GCGGTTC9350.010.5483081
CGGTTCA16200.010.0862011
GATCGGA4050.09.47384121
TGCCGAG12100.08.76058815
AGATCGG4900.08.4313920
ATCGGTT3601.714188E-47.800105630
AATGCCG17300.07.74196214
AGCGGTT9700.07.478451730
GAGCGGT8050.07.31312856
CGGAAGA9200.06.56017881
AGAGCGG9700.06.37269355
CCCGATT4806.5239455E-46.33758630
ATCGGAA4451.4791858E-66.302853622
GATCGGT3250.00269933076.12456529
ACCGATC2900.0027805066.108391322
AAGAGCG11200.05.65073444
Produced by FastQC (version 0.11.5)