FastQCFastQC Report
Fri 8 Mar 2019
C04-CTCF-EVAN_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC04-CTCF-EVAN_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7673577
Sequences flagged as poor quality0
Sequence length35-80
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAATCTCGTA483950.63067067679128Illumina Paired End PCR Primer 2 (97% over 37bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126250.1645256182351464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)